The rivals played out a 1-1 draw in regulation time, with Hasaacas Ladies winning 5-4 in the ensuing penalty shootout.
Sonia Opoku opened the scoring for Ampem Darkoa Ladies in the first half following a sustained period of dominance.
However, they could not hold on to the lead as Hasaacas Ladies returned strongly for the second half.
Having been on the front foot for a while, winger Jafaru Rahama struck to restore parity for Yusif Basigi’s side, who went on to win on penalties.
It has been a hugely successful year for Hasaacas Ladies, who have now won an unprecedented five trophies in 2021.
The Takoradi-based side had already won the Ghana Women’s Premier League, FA Cup, WAFU Zone B title and Ghana Super Cup.
Hasaacas Ladies also narrowly missed out on winning the maiden edition of the CAF Women’s Champions League, where they finished as runners-up to South African side Mamelodi Sundowns Ladies.