Hazeltine's hecklers will face ejection on a thrilling day of Ryder Cup singles after personal insults marred Saturday's action.
The Professional Golfers Association of America has demanded a "zero-tolerance" approach towards unruly fan behaviour at the Ryder Cup on the final day of action.
Rory McIlroy was targeted walking between the seventh and eighth holes on Saturday at Hazeltine by a heckler who goaded the Northern Irishman over his relationship with tennis star Caroline Wozniacki.
Henrik Stenson was interrupted on the 11th green as he lined up a putt, actions admonished by Team USA's Jordan Spieth and Patrick Reed, who went on to beat the Open champion and Justin Rose 2 and 1 in Saturday's fourballs to give the Americans a 9.5 - 6.5 lead heading into Sunday's singles.
And the PGA of America want to avoid attention being drawn away from what promises to be an absorbing final day of action on the course, despite Europe's players largely playing down the heckling's effect on their game.
A statement read: "Rooted in the ideals of sportsmanship and international goodwill, the Ryder Cup is about having a spirited passion for not only the victory, but for the game of golf.
"We are encouraging all spectators on Sunday to be passionate and support their team in a way that is respectful to those around them, the players, and the Ryder Cup.
"Our security staff will continue to enforce a zero tolerance policy, removing from the course any fans who are disruptive in any way, including the use of vulgar or profane language directed at the players.
"We look forward to a strong finish today that reflects the spirit of the Ryder Cup and provides a positive experience for all."