The match everybody wanted to see will kick off Sunday's singles at Hazeltine, with Rory McIlroy and Patrick Reed going head-to-head.
Sunday's decisive singles at the Ryder Cup will begin with a blockbuster showdown between Patrick Reed and Rory McIlroy.
Captains Davis Love III and Darren Clarke have each opted to send out their talisman in the opening match, creating a mouthwatering tie between two players who have excelled at Hazeltine this week.
Reed has claimed two and a half points from a possible four so far, in partnership with Jordan Spieth, and hit new heights in almost single-handedly beating Henrik Stenson and Justin Rose in the Saturday fourballs as the USA opened up a 9.5 - 6.5 lead.
World number three McIlroy, meanwhile, has claimed three wins in succession alongside Thomas Pieters after losing in the company of Andy Sullivan on Friday morning. He and Reed are due out at 11:04am local time (5:04pm BST).
The USA require just five points on Sunday to regain the Ryder Cup for the first time since 2008.
Phil Mickelson, in his 11th Ryder Cup appearance, will face Sergio Garcia in another notable singles battle. The duo met on the final day of the 2004 event at Oakland Hills, with the Spaniard prevailing 3 and 2.
Spieth and Stenson face off in the second match, with their contest followed by a meeting between JB Holmes and European rookie Pieters, who has enjoyed a wonderful debut tournament.
Dustin Johnson has been drawn against Chris Wood, while the anchor match brings together Zach Johnson and Matt Fitzpatrick.
Ryder Cup singles draw in full:
Patrick Reed v Rory McIlroy - 11:04am ET
Jordan Spieth v Henrik Stenson - 11:15am
JB Holmes v Thomas Pieters - 11:26am
Rickie Fowler v Justin Rose - 11:37am
Jimmy Walker v Rafael Cabrera Bello - 11:48am
Phil Mickelson v Sergio Garcia - 11:59am
Ryan Moore v Lee Westwood - 12:10pm
Brandt Snedeker v Andy Sullivan - 12:21pm
Dustin Johnson v Chris Wood - 12:32pm
Brooks Koepka v Danny Willett - 12:43pm
Matt Kuchar v Martin Kaymer - 12:54pm
Zach Johnson v Matt Fitzpatrick - 1:05pm